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. 2011 Sep 20;5(3):032004–032004-10. doi: 10.1063/1.3609262

Figure 4.

Figure 4

Relative ratios of blood cells and HIV viral particles retrieved from the top outlet. The biological samples were injected into the top inlet and PBS (for experiments containing blood) or PBS/1% BSA (for blood free experiments) was injected into the bottom. Both layers had identical flow rates of either 6 μl/min (a) or 3 μl/min (b). (a) At a flow rate of 6 μl/min, 87.3 ± 6.3% of HIV viral particles were retrieved from the top outlet in the absence of blood cells. When HIV particles were spiked in blood diluted ten times by PBS, 68.2 ± 3.3% of viruses and 12.8 ± 4.4% of blood cells were retrieved from the top outlet. In whole blood, 44.4 ± 8.7% of viruses and 19.2 ± 8.2% of blood cells were retrieved from the top outlet. (b) When the flow rate was 3 μl/min for both layers, 84.2 ± 5.8% of HIV viral particles were retrieved from the top outlet in the absence of blood cells. When HIV particles were spiked in blood diluted ten times by PBS, 64.5 ± 6.0% of viruses and 11.6 ± 6.3% of blood cells were retrieved from the top outlet. In whole blood, 46.5 ± 4.7% of viruses and 35.7 ± 4.5% of blood cells were retrieved from the top outlet.