Figure 4. Anti-IFNγ and TNF soluble receptor p75 strongly reduce epithelial CXCL10 mRNA expression but only partially attenuate epithelial NOS2 expression induced by T cells.

HNEC were co-cultured with activated T cells in the absence or presence of anti-IFNγ, TNF soluble receptor (srTNF) or an isotype-matched antibody (IgG). CXCL10 (A) and NOS2 (B) mRNA were measured by real-time PCR. Data are presented as fold change relative to those obtained in the absence of neutralizing agents. Shown are the boxplots with the 25^th, 50^th (median), 75^th percentiles, the mean (open square), the maximum and the minimum values. *p<0.05 compared with isotype matched IgG treated cultures. #p<0.05 compared with anti-IFNγ and srTNF treated cultures.