Table 1. Summary of probes introduced into FISH analysis.
Probesa | Sequences (5′– 3′) | Target nucleic acid | Aligned position |
DNA-UniC-0512-A-18 | GWATTACCGCGGCKGCTG | cytoplasmic SSU RNA | 512–529 |
DNA-UniR-0499-S-18 | CAGCMGCCGCGGUAAUWC | ||
DNA -Pdon-0587(P. donghaiense)-A-22 | TTTGGCACCTTGGAGATCTCGG | cytoplasmic LSU RNA | 587–608 |
DNA -Pdon-0602(P. donghaiense)-A-23 | ATCTCGGCTTGGCCTGCCACAGT | cytoplasmic LSU RNA | 602–624 |
DNA-Pdon-0512(P. donghaiense)-A-19 | CTTGTCTTCGGGTGAGTGA | cytoplasmic LSU RNA | 512–530 |
DNA -Pdon-0443(P. donghaiense)-A-19 | TCCTGATCGTCTCCTGCCT | cytoplasmic LSU RNA | 443–461 |
DNA -Pdon-1704(P. donghaiense)-A-23 | GGACCTGGACGAACGCCTTTCAA | cytoplasmic SSU RNA | 1704–1726 |
DNA-Pdon-0159(P. donghaiense)-A-25 | CCACTCAGAACAAATTGGAACATAC | nuclear ITS DNA | 159–183 |
DNA-Pdon-0498(P. donghaiense)-A-21 | GCCCGACAACAAGACAACAGA | nuclear ITS DNA | 498–518 |
PNA -Pdon-0443(P. donghaiense)-A-19 | TCCTGATCGTCTCCTGCCT | cytoplasmic LSU RNA | 443–461 |
Probe names follow the nomenclature outlined by Wheeler Alm et al. [51], with little revision. The first four letters stand for the kind of probe; for example, PNA stands for PNA probe. The second four-letter code is for the target of the probe. The next number is the 5′ position of the probe relative to either Escherichia coli or target organism (P. donghaiense). The next letter is for whether the probe is identical to the DNA sense or antisense strand. The last number is the length of the probe.