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. 2011 Oct 14;6(10):e25847. doi: 10.1371/journal.pone.0025847

Figure 7. Inhibition of the Hedgehog pathway leads to decreased ostegenesis in vitro.

Figure 7

(A–B) qRT-PCR of e13.5 wild-type, CD-1 palate cultures treated with DMEM F12 +/− supplementation with cyclopamine (20 mM) or Shh-N (250 ng/mL) for 2 days. N = 3, *p<0.01. (A) Wild-type, CD-1 palates treated with cyclopamine for 2 days exhibited a decrease in osteogenic markers Alp, Runx2, and Col1a1 by qRT-PCR, with a significant decrease in Col1a1. (B) Wild-type palates treated with Shh-N exhibited significant increases in the osteogenic genes Alp, Runx2, and Col1a1.