Table 1. Quantitative PCR Genes and Primer Sequences.

Gene Name	Forward primer sequence (5′ to 3′)	Reverse primer sequence (5′ to 3′)
Alkaline Phosphatase	GTTGCCAAGCTGGGAAGAACAC	CCCACCCCGCTATTCCAAAC
Gapdh	AGGAGTATATGCCCGACGTG	TCGTCCACATCCACACTGTT
Gli1	TCGACCTGCAAACCGTAATCC	TCCTAAAGAAGGGCTCATGGTA
Ihh	GCTTCGACTGGGTGTATTACG	GCTCGCGGTCCAGGAAAAT
Ptc1	GCCAAGCCCTAAAAAAAT	ACCACAATCAATCTCCTG
Runx2	CGGTCTCCTTCCAGGATGGT	GCTTCCGTCAGCGTCAACA
Shh	AAAGCTGACCCTTTAGCCTA	TTCGGAGTTTCTTGTGATCTTCC
Type I Collagen	AACCCGAGGTATGCTTGATCT	CCAGTTCTTCATTGCATTGC