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. 2011 Oct 14;6(10):e26206. doi: 10.1371/journal.pone.0026206

Figure 3. The subcellular localization of three orphan domain HHT1-causing mutants (V125D, P165L and I271N).

Figure 3

HeLa cells were transiently transfected with the C-terminally HA-tagged Endoglin pCMV5 plasmids (panels A–C, G–I and M–O) or co-transfected with the same plasmids and the EGFP-tagged H-Ras plasmid (Panels D–F, J–L and P–R) and processed for fluorescence confocal microscopy as described in the methods. In all three cases, the mutants co-localized with the ER marker (A–C for V125D; G–I for P165L and panles M–O for I271N) but not to the plasma membrane (D–F, J–L and P–R for V125D, P165L and I271N, respectively).