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. 2011 Oct 14;6(10):e26379. doi: 10.1371/journal.pone.0026379

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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NIH3T3 cells transfected with GFP-tubulin and either Cx43dT-DsRed or Cx43FL-DsRed were examined by two colour TIRF imaging. In Cx43FL-DsRed expressing cells, microtubules were centrally organized around a single MTOC (asterisk in A), while in cells expressing Cx43dT-DsRed, microtubules appear disorganized in distribution (B). Time-lapse TIRF imaging shows Cx43FL-DsRed (arrowhead in C) being transported to the cell membrane along microtubules (arrows in C) (see Movie S3). Also observed is microtubule polymerization and targeting (labeled 1-3 in D) to Cx43FL-DsRed plaques in the cell membrane (labeled a, b in D) (see Movie S4). Such microtubule targeting events were significantly decreased in NIH3T3 cells expressing Cx43dT-DsRed (n = 18) when compared with those expressing Cx43FL-DsRed (n = 16) (E). Data presented as mean ± SEM. All scale bars represent 5 µm.