Table 1.

Novel primers and reaction conditions used in the study on FQ-resistant E. coli of animal origin^a

Target	Primer directionb	Sequence (5′-3′)	Annealing temp (°C)	Amplicon size (bp)
16S rRNA for qRT-PCR	F	GGC CGC AAG GTT AAA ACT CAA ATG	52–54	243
	R	AAC CGC TGG CAA CAA AGG ATA AGG
Regulatory genes
acrR	F	CTT GTT GGG CCT GTT TGT CGT CAC	60	1,147
	R	GCT TTT GTC GGC AGA TCA CCA TTC
marRAB	F	GTA CAC CGA GGC CAT CAA CGA CT	60	1,543
	R	GAC CGG GCT GTA TAT CAA TGT A
rob	F	ACT GGG ATG CCT GGT GA	53	1,172
	R	CAA GCC CTA AAA CAT ACT CTA CTA
soxRS	F	TTA AAA ACG ATC GCT GAA GG	53	1,132
	R	TTG ACG TCG GGG GAA ACC

^aAll primers were designed in this study.

^bF, forward; R, reverse.