Table 2.
Setup of DNA stable-isotope-probing experiment with biofilm material obtained from simulated creek ecosystemsa
| Time of incubation, labeling | No. of samples of biofilm starting material from: |
|||||
|---|---|---|---|---|---|---|
| Leutra South (100 μM NH4Cl) |
Ammerbach (1,000 μM NH4Cl) |
|||||
| 13°C | 20°C | 30°C | 13°C | 20°C | 30°C | |
| 3-Week incubationb | ||||||
| NaH13CO3 | Single sample | Single samplec | Single sample | Single sample | Single samplec | Single sample |
| 6-Week incubation | ||||||
| NaH13CO3 | Duplicate | Duplicate | Duplicate | Duplicate | Duplicate | Duplicate |
| Unlabeled control | Duplicatec | Duplicate | Duplicate | Duplicate | Duplicate | Duplicate |
a
Previously studied; see reference 30.
b
No control was included for these treatments.
c
Sample was lost during analysis.