Fig. 4.

Maximum-intensity z-axis projections of representative image stacks taken from biofilms comprised of strain 30-84red with 30-84green (A), 30-84ZNgreen (B), and 30-84GacAgreen (C) after 6, 24, and 72 h. Initially, 30-84ZNgreen and 30-84GacAgreen were significantly reduced in attachment compared to 30-84green. Thus, initial attachment of 30-84green (6 h) is enhanced relative to the phenazine-deficient mutants. By 72 h, biofilms containing phenazine structural mutant 30-84ZN had formed thin, undifferentiated biofilms without distinct microcolonies, whereas biofilms containing 30-84GacA formed thicker, more extensive biofilms than the wild type alone.