Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| P. chlororaphis | ||
| 30-84 | Wild type, Rifr | W. Bockus, personal communication |
| 30-84GacA | gacA::Kmr insertion, Phz− Rifr | 2 |
| 30-84ZN | phzB::lacZ genomic fusion, Rifr | 31 |
| 30-84green | Wild type containing pKI2CM-EGFP; gfp+ | This study |
| 30-84red | Wild type containing pKI2CM-DsRed; rfp+ | This study |
| 30-84GacAgreen | 30-84GacA containing pKI2CM-EGFP; gfp+ | This study |
| 30-84GacAred | 30-84GacA containing pKI2CM-DsRed; rfp+ | This study |
| 30-84ZNgreen | 30-84ZN containing pKI2CM-EGFP; gfp+ | This study |
| 30-84ZNred | 30-84ZN containing pKI2CM-DsRed; rfp+ | This study |
| E. coli | ||
| DH5α | F−recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(argF-lacZYA)I169 φ80lacZ ΔM15 λ− | GIBCO-BRL |
| HB101 | F−hsdS20(rB− mB−) supE44 recA1 ara14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-5 λ− | GIBCO-BRL |
| Plasmids | ||
| pRK2013 | ColE1, Kmr | Clontech |
| pProbe-KI′ | pVS1 replicon, p15a origin of replication, inaZ transcriptional fusion; Kmr | 25 |
| pEGFP | ColE1, contains an enhanced variant of the Aequorea victoria green fluorescent protein (EGFP); Apr Kmrgfp+ | Clontech |
| pDsRed-Express | ColE1, contains Discosoma sp. red fluorescent protein (DsRed-Express); Apr Kmrrfp+ | Clontech |
| pHP24Ω-Cm | Interposon, contains cat; Cmr | 8 |
| pKI2CM-EGFP | pPROBE-KI′ containing the 4.3-kb EcoRI fragment carrying cat and a 974-bp PvuII-EcoRI fragment carrying EGFP; Kmr Cmrgfp+ | This study |
| pKI2CM-DsRed | pPROBE-KI′ containing the 4.3-kb EcoRI fragment carrying cat and a 930-bp PvuII-EcoRI fragment carrying DsRed-Express; Kmr Cmrrfp+ | This study |
a
Apr, Cmr, Kmr, and Rifr indicate resistance to ampicillin, chloramphenicol, kanamycin, and rifampin, respectively.