Table 2.
Biofilm characteristics for representative biofilms formed by wild-type strain 30-84 with itself and each mutant after 96 h
| Biofilm parameter | Result fora: |
||
|---|---|---|---|
| 30-84 + 30-84 | 30-84 + 30-84GacA | 30-84 + 30-84ZN | |
| Biovolume (μm3/μm2) | 0.474 ± 0.112 B | 0.990 ± 0.124 A | 0.299 ± 0.071 C |
| Thickness (μm) | 2.811 ± 0.453 B | 6.334 ± 1.190 A | 2.180 ± 0.643 B |
| Diffusion distance (μm)b | 0.007 ± 0.002 B | 0.013 ± 0.001 A | 0.001 ± 0.000 C |
| No. of microcoloniesc | 12.200 ± 4.716 B | 31.000 ± 3.493 A | NAd |
| Microcolony area (μm2) | 50.879 ± 7.550 A | 64.584 ± 8.430 A | NA |
a
The numbers reported are averages ± standard errors, and the letters following the values indicate significant differences.
b
Diffusion distance is defined as the minimum distance between a pixel of biovolume and a pixel of void space. Higher values thus indicate larger aggregates of biovolume.
c
Microcolonies were defined as aggregates that occupy an area of at least 25 μm2 in a maximum-intensity vertical projection of image stacks.
d
NA, microcolony counts and sizes are not reliable in the case of the relatively undifferentiated biofilms formed by 30-84 + 30-84ZN.