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. 2011 Oct;77(20):7271–7278. doi: 10.1128/AEM.06022-11

Fig. 2.

Fig. 2.

RFLP analysis of anaC amplicons (366 bp) of cyanobacterial strains and environmental samples with the HhaI and HinfI restriction enzymes. The amplicons are indicated above the lanes according to the strain/sample used for amplification: Anabaena sp. 37 (A37), Oscillatoria sp. PCC 6506 (6506), Oscillatoria sp. PCC 9107 (9107), and Aphanizomenon sp. 3 (Aph). Lake Pyhäjärvi (L. Pyh), Lake Kymijärvi (L. Kym), and Baltic Sea Helsinki (BSH) samples were amplified directly from environmental DNA, while cBSH.1 and cBSH.2 indicate the cloned anaC fragments of the BSH sample. ud, digestion reactions without the enzyme; SM, size marker. Asterisks indicate the 29-bp fragment detected in HhaI digestion. The panel for each enzyme is assembled from two separate gel images.