Table 3.
Anatoxin-a and homoanatoxin-a production as determined by LC-MS and detection of the anaC gene by PCR in the cyanobacterial strains studied
| Strain | Toxina | PCRb |
|||
|---|---|---|---|---|---|
| General primers |
Genus-specific primers |
||||
| anxgen | anaC-gen | anaC-anab | anaC-osc | ||
| Anabaena sp. | |||||
| 14 | ana | + | + | + | − |
| 37 | ana | + | + | + | − |
| 54 | ana | + | + | + | − |
| 86 | —c | + | + | + | − |
| 130 | ana | + | + | + | − |
| Oscillatoria sp. | |||||
| 193 | ana | + | + | − | + |
| PCC 6407 | NAc | + | + | − | + |
| PCC 6412 | NAc | + | + | − | + |
| PCC 6506 | hana | + | + | − | + |
| PCC 9029 | hana | + | + | − | + |
| PCC 9107 | NAc | − | + | − | + |
| PCC 9240 | NAc | + | + | − | + |
| PCC 10111 | hana | − | + | − | + |
| PCC 10601 | NAc | + | + | − | + |
| PCC 10608 | —c | +d | − | − | − |
| PCC 10702 | —c | − | − | − | − |
| Aphanizomenon sp. 3 | —c | + | + | − | − |
| Anabaena sp. 90 | mc | − | − | − | − |
a
ana, anatoxin-a; hana, homoanatoxin-a; —, no toxin production detected; NA, not analyzed; mc, microcystin-producing strain (49).
b
Primer pair anaC-anab was specific for Anabaena strains, and primer pair anaC-osc was specific for Oscillatoria strains. +, amplification product detected by PCR; −, no amplification by PCR.
c
Anatoxin-a or homoanatoxin-a was detected previously by Sivonen et al. (47), Aráoz et al. (4), or Cadel-Six et al. (7).
d
The annealing temperature was 50°C instead of 52°C.