Table 5.
Detection of the anaC gene in environmental samples by PCR and RFLP analysis
| Samplea | Toxinb | PCRc |
RFLP patternd | |||
|---|---|---|---|---|---|---|
| Genus-specific primers |
General primers |
|||||
| anaC-anab | anaC-osc | anxgen | anaC-gen | |||
| Lake Pyhäjärvi | epohana | (+) | − | − | (+) | OscI |
| Lake Kymijärvi | hana | − | + | + | + | OscII |
| Lake Kirkkojärvi | dhana | − | + | − | (+) | NA |
| Lake Kyrösjärvi | ana | − | + | − | (+) | NA |
| Lake Ranuanjärvi | dhana, ana | + | + | − | (+) | NA |
| River Ruskonjoki | ana | (+) | (+) | − | (+) | NA |
| Baltic Sea, Halikko | epohana | (+) | − | − | (+) | NA |
| Baltic Sea, Luvia | dhana | − | − | − | (+) | NA |
| Baltic Sea, Helsinki | NA | + | + | + | + | Ana, OscII |
a
All environmental samples were taken from locations in Finland.
b
ana, anatoxin-a; hana, homoanatoxin-a; epohana, epoxyhomoanatoxin-a; dhana, dihydroanatoxin-a; NA, not analyzed.
c
+, amplification product detected; (+), weak amplification; −, no amplification.
d
OscI, HhaI RFLP pattern identical to that of Oscillatoria sp. PCC 6506; OscII, HhaI RFLP pattern identical to that of Oscillatoria sp. PCC 9240; Ana, RFLP pattern identical to that of Anabaena strains (see Table 4 for details).