Table 3.
Chlortetracycline sensitivities of Megasphaera elsdenii isolates from organically raised and feral swinea
Swine origin | No. of M. elsdenii isolates growing at chlortetracycline concn (μg/ml): |
||
---|---|---|---|
0 | 16 | 64 | |
Organic | 540 | 355 (66%) | 264 (52%) |
Feral | 91 | 0 | 0 |
M. elsdenii population levels averaged 2.4 × 108 CFU/g (range, 1.2 × 108 to 4 × 108) in organically raised swine. M. elsdenii cultivable populations in feral swine were significantly lower than those in organically raised animals, averaging 1.5 × 106 CFU/g (P < .001, CI = 95%, Welch's t test), and also more variable (range, <5 × 103 to 2 × 107 CFU/g). Chlortetracycline susceptibilities of M. elsdenii cloned isolates were determined by replica-patching colonies sequentially onto PYG agar plates containing 64, 16, and 0 μg chlortetracycline per ml (18a). Values in parentheses indicate resistant isolates expressed as percentages of totals tested.