Table 5.

Experiment B: SNP-based identification of Megasphaera elsdenii isolates from swine fecal samples^a

Animal	M. elsdenii treatment	Day after birth	No. of M. elsdenii isolates tested	SNP-based identityb
				Inoculated strain					Sow isolate	Unique isolate
				F101	F103	24-50C	26-50	33-54
Sow	NAd	NA	125	0	0	0	0	0	125	NA
Offspring (5 pigs)	Five strains inoculated	10	53	0	8	0	45	0	0	0
	intragastrically	18	47	0	0	0	35	12	0	0
		31	83	0	0	0	64	19	0	0
		45	93	0	0	0	28c	57	7	1
		73	92	0	0	0	2	18	52	20
Offspring (4 pigs)	Sterile medium (control)	10	17	0	15	2	0	0	0	0
		18	0
		31	69	0	0	0	6	0	34	29
		45	93	0	0	0	0	20	63	10
		73	89	0	0	0	3	2	83	1

^aBased on comparisons of hgd-odt sequences (1,108 nucleotides).

^bA unique isolate did not match any of the five inoculum mixture strains or any of the 125 sow strains.

^cThree isolates from two pigs had the SNP pattern of M. elsdenii strain 26-50 and were tylosin resistant (Table 4).

^dNA, not applicable.