Table 3.
Reproducibility of populations associated with large particles from both the Fract-2006 and the Particle-2007 studies
| Populationa | AdaptML prediction |
Statistical comparison |
||
|---|---|---|---|---|
| Fract-2006b | Particle-2007c | P valued | Reproducede | |
| V. fischeri | Y | S | 0.85 | + |
| V. splendidus cluster 2 (F) | mixed | S | 0.64 | + |
| V. breoganii | Y | S | 0.57 | + |
| V. splendidus cluster 2 (S) | mixed | S | 0.55 | + |
| V. splendidus cluster 1 (S) | Y | S | 0.46 | + |
| Vibrio sp. F10 | Y | S | 0.39 | + |
| Vibrio sp. F12 | Y | NS | 0.34 | + |
| V. crassostreae | Y | S | 0.23 | + |
| Vibrio sp. F5 | Y | NF | 0.16 | + |
| V. cyclitrophicus | Y | NS | 0.08 | + |
| Enterovibrio calvensis-like | N | S | 0.04 | − |
| V. tasmaniensis | N | S | 0.03 | − |
| Vibrio sp. I3 | NS | S | 0.01 | − |
Population names are given according to those presented in Fig. 1. V. splendidus was the only population sufficiently represented in the spring to allow comparisons; the spring (S) and fall (F) samples are compared separately. All other populations were tested using only fall samples.
Data indicate each populations predicted to be associated with the large particle- of a zooplankton-enriched fraction (Y) or one of alternate habitats (N) or not predicted as a significant population (NS); in the case of the large V. splendidus population, 5 of 8 subpopulations were associated with large particles (mixed).
Populations were predicted as significant (S), nonsignificant (NS), or not found (NF) in the samples consisting only of large particles and zooplankton.
Student's t test was performed to determine whether the percentages of the corresponding populations isolated in the Fract-2006 and Particle-2007 studies were statistically significantly different. The Fract-2006 study included 3 replicates in each season (spring and fall), and percentages were calculated from the total number of strains isolated from particles >64 μm in size. The particle-2007 study included 8 replicates in each season. P values were calculated from the fall sample data unless otherwise noted in the population column.
Populations were considered to have been reproduced (+) if the percentages of the corresponding populations of all strains isolated from large particles were not statistically significantly different across studies (P < 0.05).