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. 2011 Nov;193(21):6057–6069. doi: 10.1128/JB.05671-11

Fig. 2.

Fig. 2.

Type VI-dependent secretion of Hcp (SMA2263). (A) Anti-Hcp immunoblot of secreted and cellular proteins produced by wild-type S. marcescens Db10 (WT) and T6SS mutant strains SJC10 (Δlip), SJC3 (ΔclpV), SJC11 (ΔtssE), SJC12 (ΔicmH), and SJC4 (ΔSMA2254-2274). The amount of sample loaded for each fraction corresponded to the number of cells. (B) Anti-Hcp immunoblot of secreted proteins produced by wild-type S. marcescens Db10, T6SS mutants, and T6SS mutants carrying either the control plasmid (pSUPROM, Vector) or a plasmid supplying the missing component in trans (pSC039, ClpV; pSC066, Lip; pSC045, TssE). Hcp (SMA2263) is indicated by a black arrow.