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. 2011 Nov;193(21):6057–6069. doi: 10.1128/JB.05671-11

Fig. 3.

Fig. 3.

Action of the S. marcescens Db10 type VI secretion system against C. elegans and E. coli. (A) Killing curves comparing the life span of C. elegans grown on wild-type (WT) S. marcescens Db10 and on T6SS mutant strains SJC3 (ΔclpV, left) and SJC4 (ΔSMA2254-2274, right) at the temperature indicated. For each strain, n=80 worms, error bars show standard errors of the means (SEM), and in neither case was the difference between the strains statistically significant (P > 0.05 by log rank test). (B and C) Recovery of viable E. coli MC4100 cells after 4 h of coculture with the S. marcescens strain indicated at 37°C, with an initial ratio of 5 S. marcescens cells to 1 E. coli cell (see Materials and Methods for full details). (B) Comparison of wild-type S. marcescens Db10 with T6SS mutant strains SJC10 (Δlip), SJC11 (ΔtssE), SJC3 and SJC4; inoculum, starting number of E. coli cells at t=0 h. (C) Comparison of wild-type S. marcescens Db10 and T6SS mutants carrying either the control plasmid (pSUPROM, Vector) or a plasmid supplying the component in trans (pSC066, Lip; pSC045, TssE). In all cases, points show means ± SEM (n ≥ 3).