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. 2011 Nov;193(21):6057–6069. doi: 10.1128/JB.05671-11

Fig. 4.

Fig. 4.

Action of the S. marcescens type VI secretion system against competitor bacteria Pseudomonas fluorescens and Enterobacter cloacae. Recovery of viable P. fluorescens 55 (A and B) or Enterobacter cloacae ATCC 13047 (C) cells after 4 h of coculture with the S. marcescens strain indicated at 30°C, with an initial ratio of 5 S. marcescens cells to 1 competitor cell (see Materials and Methods for full details). (A and C) Comparison of wild-type S. marcescens Db10 (WT) with T6SS mutant strains SJC3 (ΔclpV) or SJC4 (ΔSMA2254-2274); inoculum, starting number of competitor cells at t=0 h. (B) Comparison of wild-type S. marcescens Db10 and T6SS mutants SJC3 and SJC11 (ΔtssE) carrying either the control plasmid (pSUPROM, Vector) or a plasmid supplying the component in trans (pSC039, ClpV; pSC045, TssE). (D) Survival of wild-type (WT) and T6SS mutant strains of S. marcescens Db10 after coculture with Enterobacter cloacae at an initial ratio of 1:1. The ratio of S. marcescens to Enterobacter cloacae cells recovered is reported after 4 h at 30°C. Throughout, points show means ± SEM (n ≥ 4).