Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Serratia marcescens Db10 | Wild-type strain, nonpigmented | 16 |
| SJC3 | Db10 ΔclpV (ΔSMA2274) in frame | This study |
| SJC4 | Db10 ΔSMA2254-2274 (ΔicmH-clpV) | This study |
| SJC10 | Db10 Δlip (ΔSMA2252) in-frame | This study |
| SJC11 | Db10 ΔtssE (ΔSMA2271) in-frame | This study |
| SJC12 | Db10 ΔicmH (ΔSMA2254) in-frame | This study |
| SLM1 | Db10 ΔlacZ (ΔSMA2462) in-frame | This study |
| SLM8 | SLM1 with lacZ inserted between clpV and SMA2275 to generate nondisruptional transcriptional reporter (SMA2274-lacZ-SMA2275) | This study |
| SLM9 | SLM1 with lacZ replacing clpV to generate T6S-inactive transcriptional reporter (ΔSMA2274::lacZ) | This study |
| Serratia marcescens ATCC 274 | Wild-type strain | Lab stock |
| SJC17 | Smr derivative of ATCC 274 | This study |
| Escherichia coli MC4100 | Model laboratory E. coli K-12 strain; Smr (rpsL150) | 8 |
| Escherichia coli DH5a | Cloning host | Invitrogen |
| Escherichia coli CC118λpir | Cloning host and donor strain for pKNG101-derived marker exchange plasmids (λpir) | 20 |
| Escherichia coli HH26 pNJ5000 | Mobilizing strain for conjugal transfer | 18 |
| Escherichia coli S17-1λpir (pUT-miniTn5 Sp/Sm) | Donor strain of miniTn5 Sp/Sm transposon | 13 |
| Escherichia coli S17-1λpir (pUT-miniTn5 Kn) | Donor strain of miniTn5 Kn transposon | 13 |
| Enterobacter cloacae ATCC 13047 | Wild-type strain (Smr) | ATCC |
| Pseudomonas fluorescens 55 | Wild-type strain | SCRI collection |
| KT02 | Smr derivative of P. fluorescens 55 | This study |
| KT03 | Knr derivative of P. fluorescens 55 | This study |
| Plasmids | ||
| pSUPROM | Vector for constitutive expression of cloned genes under the control of the E. coli tat promoter (Kanr) | 24 |
| pBluescript KS+ | High-copy-number cloning vector (Apr) | Stratagene |
| pKNG101 | Suicide vector for marker exchange (SmrsacBR mobRK2 ori R6K) | 26 |
| pSC006 | pKNG101-derived marker exchange plasmid for the generation of chromosomal ΔSMA2274 | This study |
| pSC008 | pKNG101-derived marker exchange plasmid for the generation of chromosomal ΔSMA2254-2274 | This study |
| pSC039 | clpV (SMA2274) coding sequence in pSUPROM | This study |
| pSC040 | pKNG101-derived marker exchange plasmid for the generation of chromosomal ΔSMA2271 | This study |
| pSC041 | pKNG101-derived marker exchange plasmid for the generation of chromosomal ΔSMA2254 | This study |
| pSC042 | pKNG101-derived marker exchange plasmid for the generation of chromosomal ΔSMA2252 | This study |
| pSC045 | tssE (SMA2271) coding sequence in pSUPROM | This study |
| pSC066 | lip (SMA2252) coding sequence in pSUPROM | This study |
| pSC1003 | pKNG101-derived marker exchange plasmid for the generation of chromosomal ΔSMA3462(ΔlacZ) | This study |
| pSC1016 | pKNG101-derived marker exchange plasmid for the generation of chromosomal ΔSMA2274::lacZ | This study |
| pSC1017 | pKNG101-derived marker exchange plasmid for the generation of chromosomal SMA2274-lacZ-SMA2275 | This study |
| pET-2263N | SMA2263 (Hcp) with N-terminal, TEV-cleavable hexa-histidine tag in pET15b (Novagen) | This study |