Fig. 1.

Accumulation of the flagellin Hag in the ymdB deletion strain. The proteome of a ymdB mutant strain was compared to the wild-type strain. Crude extracts were isolated from B. subtilis strains grown in complex medium (LB) at 37°C. Lane 1, 168 (wild type); lane 2, GP583 (ΔymdB); lane 3, GP904 (ΔymdB Δhag); lane 4, GP925 (ΔymdB complementation with pGP1062); lane 5, protein weight marker. Fifteen micrograms of crude extract of each culture was loaded on a 12% sodium dodecyl sulfate-polyacrylamide gel. After electrophoresis the gel was stained with Coomassie brilliant blue G-250. The flagellin Hag is indicated with an arrow.