Fig. 6.

The cellular amount of SigD is increased in a ymdB mutant. Crude extracts were isolated from the B. subtilis strains GP948 (sigD-FLAG; wild type) and GP950 (sigD-FLAG; ΔymdB strain) grown in complex medium (LB) at 37°C. Five micrograms of crude extract of each culture was loaded on a 12% sodium dodecyl sulfate-polyacrylamide gel. After electrophoresis and blotting onto a polyvinylidene difluoride membrane, the FLAG tag was detected using rabbit polyclonal antibodies. All cultures were harvested in the stationary phase. As a loading control, the membrane was stained with Coomassie brilliant blue G-250. α, anti.