Table 2.
Plasmids
| Plasmid | Relevant characteristic(s) | Reference |
|---|---|---|
| pZep08 | GFP reporter plasmid; Apr Cmr | 34 |
| pZep-proU-1 | Positions −270–+230 of PproU cloned into pZep08 | This study |
| pZep-proU-2 | Positions −240–−80 of PicsB cloned into pZep-proU-1 in the reverse orientation | This study |
| pZep-proU-3 | Positions −240–−80 of PicsB cloned into pZep-proU-1 in the forward orientation | This study |
| pZep-proU-4 | Positions −150–+230 of the PproU promoter cloned into pZep08 | This study |
| pZep-proU-5 | Positions −240–−80 of PicsB cloned into pZep-proU-4 in the reverse orientation | This study |
| pZep-proU-6 | Positions −60–+230 of the PproU promoter cloned into pZep08 | This study |
| pZep-proU-7 | Positions −240–−80 of PicsB cloned into pZep-proU-8 in the reverse orientation | This study |
| pZep-proU-8 | Positions −240–−80 of PicsB cloned into pZep-proU-6 in the reverse orientation with the URE upstream | This study |
| pZep-proU-9 | 210-bp yaeT coding region cloned into pZep-proU-6 | This study |
| pZep-proU-10 | Positions −240–−80 of PicsB cloned into pZep-proU-9 in the reverse orientation | This study |