Skip to main content
. 2011 Nov;193(21):5985–5996. doi: 10.1128/JB.05869-11

Table 3.

Expression of lac fusions in different argP variant derivativesa

graphic file with name zjb9990909200004.jpg

a

Derivatives of the ΔargP strain GJ9602 each carrying two plasmids, as follows, were used in the experiments. (i) Plasmid vector pCL1920 (Δ) or its derivatives with argP+ (+; pHYD915) or the different argPd variants indicated. (ii) One of the following lac fusion plasmids: pHYD1723 (argO), pHYD2660 (argT), pHYD2668 (asd), pHYD2669 (dapB), pHYD2610 (dapD), pHYD2671 (dnaA), pHYD2602 (gdhA), pHYD2670 (lysA), pHYD2664 (lysC), pHYD2636 (lysP), or pHYD2672 (nrdA).

b

Specific activities of β-galactosidase (β-Gal) after growth in glucose-minimal A medium supplemented with 18 amino acids other than Arg or Lys (Nil), 18 amino acids and 1 mM Arg (Arg), or 18 amino acids and 1 mM Lys (Lys) are reported.

c

Data indicate the degree of Lys repression for the lac fusion strain concerned, relative to Arg supplementation in the case of argO and to Nil supplementation for the rest.

d

Data indicate the degree of regulation imposed by the ArgP variant concerned (or the wild-type ArgP) relative to that in the ΔargP strain, in medium with Arg supplementation in the case of argO and with Nil supplementation for the rest.