Table 3.
Expression of lac fusions in different argP variant derivativesa
Derivatives of the ΔargP strain GJ9602 each carrying two plasmids, as follows, were used in the experiments. (i) Plasmid vector pCL1920 (Δ) or its derivatives with argP+ (+; pHYD915) or the different argPd variants indicated. (ii) One of the following lac fusion plasmids: pHYD1723 (argO), pHYD2660 (argT), pHYD2668 (asd), pHYD2669 (dapB), pHYD2610 (dapD), pHYD2671 (dnaA), pHYD2602 (gdhA), pHYD2670 (lysA), pHYD2664 (lysC), pHYD2636 (lysP), or pHYD2672 (nrdA).
Specific activities of β-galactosidase (β-Gal) after growth in glucose-minimal A medium supplemented with 18 amino acids other than Arg or Lys (Nil), 18 amino acids and 1 mM Arg (Arg), or 18 amino acids and 1 mM Lys (Lys) are reported.
Data indicate the degree of Lys repression for the lac fusion strain concerned, relative to Arg supplementation in the case of argO and to Nil supplementation for the rest.
Data indicate the degree of regulation imposed by the ArgP variant concerned (or the wild-type ArgP) relative to that in the ΔargP strain, in medium with Arg supplementation in the case of argO and with Nil supplementation for the rest.
