Table 4.
Apparent Kds of ArgP binding to cis regulatory regions of different genesa
| cis regulatory region |
Kd (nM) |
|
|---|---|---|
| −Lys | +Lys | |
| argO | 15 | 15 |
| argT | >400 | >400 |
| asd | 170 | >200 |
| dapB | 120 | >150 |
| dapD | 70 | 120 |
| dnaA | 150 | 150 |
| gdhA | 80 | 130 |
| lysA | 150 | 210 |
| lysC | 70 | 110 |
| lysP | 55 | >140 |
| nrdA | 150 | 150 |
| lacZ | >400 | >400 |
a
From the EMSA autoradiograph for each of the DNA templates, the amounts of radioactivity in the bands corresponding to free (unbound) DNA were densitometrically determined for the different lanes, and the apparent Kd in the absence (−) or presence (+) of 0.1 mM Lys was calculated from the curve-plotting values of the unbound fraction versus the ArgP concentrations used.