Table 7.
Effects of CadC, LysP, and ArgP on cadBA-lac expression
| Strain (genotype) | β-Gal sp act (Miller units)a |
|||
|---|---|---|---|---|
| pH 7.4 |
pH 5.8 |
|||
| Nil | Lys | Nil | Lys | |
| MC4100 (wild type) | 25 | 28 | 24 | 98 |
| GJ9623 (ΔlysP) | 27 | 27 | 94 | 96 |
| GJ9647 (ΔcadC) | 25 | 26 | 26 | 24 |
| GJ9602 (ΔargP) | 23 | 22 | 26 | 96 |
| GJ9649 (ΔlysP ΔcadC) | 21 | 28 | 24 | 26 |
| GJ9648 (ΔargP ΔcadC) | 25 | 26 | 24 | 28 |
| GJ9624 (ΔargP ΔlysP) | 27 | 24 | 87 | 99 |
a
Values reported are the specific activities of β-galactosidase (β-Gal) in the indicated strain derivatives carrying the cadBA-lac fusion plasmid pHYD2674 after growth in minimal A-based medium of pH 7.4 or pH 5.8 supplemented with either 19 amino acids other than Lys (Nil) or 19 amino acids and 10 mM Lys (Lys).