Table 9.
Tests for ArgP regulation of genes related to Arg transport
| Plasmid (genotype) | β-Gal sp act (Miller units)a |
||||||
|---|---|---|---|---|---|---|---|
| Wild-type strain with: |
ΔargP strain with: |
argR with Arg | |||||
| Nil | Arg | Lys | Nil | Arg | Lys | ||
| pHYD2658 (artJ-lac) | 1,353 | 286 | 1,480 | 1,256 | 231 | 1,252 | 1,700 |
| pHYD2659 (artP-lac) | 170 | 105 | 197 | 184 | 122 | 210 | 190 |
| pHYD2660 (argT-lac) | 249 | 279 | 242 | 279 | 255 | 221 | 198 |
| pHYD2661 (hisJ-lac) | 227 | 115 | 262 | 244 | 114 | 296 | 289 |
a
Values reported are the specific activities of β-galactosidase in derivatives of MC4100 (wild type), GJ9602 (ΔargP), and GJ4748 (argR) carrying the indicated lac fusion plasmid after growth in glucose-minimal A medium without (Nil) or with supplementation with Arg or Lys at 1 mM.