Table 2.
Primers used in this study
| Primer name | Sequence (5′→3′)a |
|---|---|
| 24151rtF | CCCTGGTTGATGTCGTAAGG |
| 24151rtR | TGTGATCCGCAATCATCAGT |
| 24141rt | GATGCTGATACCATAGGC |
| 24151rtR | TGTGATCCGCAATCATCAGT |
| 24151rtF | GTCCTGATTCCTCTTAAAGCAATG |
| 24161rtR | GGTGAAGAGAATAAGTGCTGTCTGG |
| 24161rt | CAAACGCATCTACTACCCAGGA |
| 24171dsrt | GAAACCCGTCACGTCAATGTT |
| 24191F | GCGTTCACTCGGGTCACC |
| 24181dsrt | CCAGTTGAAAATCCAATCACGG |
| 24111rtF | GGCAGCCCAGCATTCTACTA |
| 24111rtR | AAAGACCTCATCAGGCATGG |
| 24091rt | GGTAGCAGGCTATTCATTGAC |
| 24101rt | GGAGAGTCCTTGCTTCATGG |
| 24101dsrt | TTGGTATCGGTTGGCTGAG |
| 24111rt2 | GCATGGGCAAACCAAAC |
| 24111rt | TGCCAACAATCCTTCCTGAT |
| 2412rt2 | TGACCCACGCAGTTTAGC |
| 24121rt | TTGGAAGTCAAATCGGTTCC |
| 24131rtF | TCATTGGGCTTGATTGATGA |
| tolCF3 | GTGTTTCAACCCATGTGTTTG |
| TolC-5′R-StuI | AGGCCTGTAAAGAGCGGCACGAGC |
| TolC-3′-F2 | GATTAACTCATGGTTAGCAACT |
| TolC-R2-XbaI | AAAAAATCTAGACTTTTCCGCCAGAC |
| TolC-3′-F2-XbaI | AAAAAATCTAGACGCAATCCTTGGTA |
| TolC-5′-R2 | ATGGTATCGTTCTCCAGAGC |
| 23601F | CCCGGGTCTGAGACAAATGAATGAAAGAATGC |
| 23601R | TCTAGATTCGCTTCTATAGTATGGC |
| 27401F | TTATGGTCAAATCACGAACGC |
| 27401R | AGTTGTTTCGCTCCCTGAATA |
| 07981F | CAGGCTCAGGCGAAAGTAG |
| 07981-5′R-HpaI | GTTAACAAATCCTGCTATGAGTTCAGC |
| 07981-3′F-HpaI | GTTAACGACTCGTTATCTCCACAGGTT |
| 07981R | TGGCAACGGCACAATCACC |
| 24191Fcomp | GGTACCGCACCAGTCGATACCCTGTT |
| 24191Rcomp | TCTAGACGCAAGCCTGATTGTTACC |
| 24111Fcomp | GGTACCAAGGAGGATAGACTCGGTTTGTT |
| 24111Rcomp | TCTAGACCTCATACTCGCTATCATCATTG |
| 07981F2comp | GGCTTGTTGTTCTTTACTACC |
| 07981R3comp | GTCGCAGGTATCCACAAAGT |
| tolCF | CGTCTGCCTTGAGTGTTTC |
| tolCR | TCACATTGGATTGACTGCGT |
a
Restriction sites are underlined.