Fig. 2.
Visualization of methanol dehydrogenase activity in the xoxF and mxaF mutant strains using isoelectric focusing gel electrophoresis. Cells were grown in minimal medium containing both succinate and methanol, and cell extracts from these strains were isoelectrofocused using a pH range of 3 to 9. The presence of methanol dehydrogenase activity was detected by using nitroblue tetrazolium and phenazine methosulfate in the presence of 20 mM methanol and 15 mM ammonium chloride. xoxF12sup refers to two different xoxF1 xoxF2 suppressor mutants that were spontaneously isolated.