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Expression of mxc-xylE and mxb-xylE promoter fusions in M. extorquens AM1 strains. mxc (black) and mxb (white) promoter activities were determined by measuring catechol 2,3-dioxygenase activity (XylE) in crude cell extracts of strains grown in medium containing both succinate and methanol. Cell extracts containing the promoterless xylE construct pHX200 had a background activity of 10.9 ± 2.6 nmol min−1 mg protein−1.
