Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference(s) |
|---|---|---|
| Strains | ||
| AM1 | Rifr derivative (wild type) | 27 |
| AA31 | mxcE (moxEa); chemically induced mutation | 27 |
| CM194.1 | ΔmxaF | 22 |
| ES890 | xoxF1::Km | This study, 6b |
| ES970 | ΔxoxF2 | This study |
| ES1022 | ΔxoxF2 xoxF1::Km | This study |
| ES1100 | ΔmxaF ΔxoxF2 xoxF1::Km | This study |
| ES1126 | ΔxoxF2 xoxF1::Kmsup; spontaneously isolated suppressor mutant | This study |
| ES1258 | mxbM::Km | This study |
| ES1291 | xoxG::Km | This study |
| ES1296 | ΔxoxF2 xoxG::Km | This study |
| Plasmids | ||
| pAP3 | Expression vector using the META1_3512 promoter (Tcr) | This study |
| pAP5 | Promoterless yfp fusion vector created from pCM62 (Tcr) | This study |
| pALS24 | pHX200 with mxbDM promoter region (Tcr) | 34 |
| pAYC61 | Allelic exchange suicide vector (Tcr Apr) | 5 |
| pCM62 | Expression vector using the lac promoter (Tcr) | 20 |
| pCM80 | Expression vector using the lac and mxa promoters (Tcr) | 20 |
| pCM86 | mxaF in pCM80 (Tcr) | 20 |
| pCM157 | cre expression vector (Tcr) | 21 |
| pCM184 | Allelic exchange suicide vector (Kmr Tcr Apr) | 21 |
| pES134-135 | pCM184 with xoxF2 upstream and downstream flanks (Kmr Tcr Apr) | This study |
| pES222-223 | pCM184 with upstream and downstream xoxG flanks (Kmr Tcr Apr) | This study |
| pES501 | pHX200 with mxcQE promoter region (Tcr) | This study |
| pES500 | xoxF1, xoxG, and xoxJ cloned into pAP3 (Tcr) | This study |
| pES502 | pAP5 with mxa promoter region (Tcr) | This study |
| pES503 | pAP5 with xox1 promoter region (Tcr) | This study |
| pHX200 | Promoterless xylE fusion vector (Tcr) | 39 |
| pLC6168 | pAYC61 with xoxF1upstream and downstream flanks (Kmr Tcr Apr) | 6 |
a
The mxcE mutant strain was originally reported under the name moxE.
b
The plasmid used to construct the xoxF1::Km mutant was constructed as described previously (6) and conjugated into the wild-type M. extorquens AM1 strain background used in this study.