Table 2.
Strain | Growth with carbon source |
Complementation in methanol medium |
|||||
---|---|---|---|---|---|---|---|
MeOH | MA | Formate | pCM80b | pCM86 | pAP3c | pES500 | |
WT | ++ | ++ | + | ++ | ++ | ++ | ++ |
mxaF | − | ++ | + | − | + | − | − |
xoxF1 | + | ++ | + | ND | ND | ND | ND |
xoxF2 | ++ | ++ | + | ND | ND | ND | ND |
xoxF1 xoxF2 | − | ++ | + | − | − | − | ++ |
xoxG | ++ | ++ | + | ND | ND | ND | ND |
xoxF2 xoxG | ++ | ++ | + | ND | ND | ND | ND |
Abbreviations are as follows: MeOH, methanol; MA, methylamine; WT, wild type; ND, not determined. −, no growth; + or ++, growth (a single plus indicates that colony size is about half that seen for a wild-type strain on methanol medium).
pCM80 is the empty parent vector for pCM86, which harbors mxaF (20).
pAP3 is the empty parent vector for pES500, which harbors the xoxF, xoxG, and xoxJ genes.