Fig. 5.

Metal sensitivity associated with MntS overproduction or a lack of MntP. (A) Ten-fold serial dilutions of mid-exponential-phase cultures of wild-type (wt; MG1655) or ΔmntS (GSO459) cells bearing the pBAD24 empty vector or pBAD24-MntS were spotted onto LB plates containing 0.2% arabinose, 100 μg/ml ampicillin, and the indicated amounts of metals. The effects of 4 mM FeSO₄, 6 mM FeCl₃, 2 mM NiCl₂, 3 mM CuCl₂, 1 mM CoCl₂, and 1 mM CdSO₄ were similarly tested (data not shown). (B) Ten-fold serial dilutions of mid-exponential-phase cultures of wild-type (MG1655), ΔmntR (GSO458), ΔmntS (GSO459), ΔmntH (GSO460), and ΔmntP (GSO461) were spotted onto LB plates containing the indicated amounts of metals. The effects of 4 mM FeSO₄, 6 mM FeCl₃, 2 mM NiCl₂, and 3 mM CuCl₂ were similarly tested (data not shown).