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Lack of mntH repression in the ΔmntS strain. Primer extension analysis monitored the mntH and mntS transcript levels, and Western blot analysis was used to determine MntS-SPA expression in stationary-phase cultures of wild-type (wt; MG1655) or ΔmntS (GSO459) strains grown overnight in M9-glucose medium containing the indicated concentration of MnCl2.
