Table 1.
L. monocytogenes strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant feature(s) | Reference |
|---|---|---|
| Strains | ||
| 10403S | Wild-type (serotype 1/2a) | 5 |
| NF-L943 | prfA with G155S mutation in 10403S background (overexpresses PrfA-dependent genes) | 37 |
| DP-L1075 | 10403S prfA::Tn917-LTV3 | 13 |
| DP-L1935 | Internal in-frame deletion of plcB in 10403S background | 39 |
| DP-L2296 | Internal in-frame deletion of mpl in 10403S background | 26 |
| DP-L2343 | Deletion of mpl structural gene in 10403S background | 36 |
| DP-L5596 | 10403S prsA2::Himar1 | 47 |
| DP-L5601 | Internal in-frame deletion of prsA2 from positions 29 to 291 in 10403S background (ΔprsA2) | 47 |
| DP-L5603 | DP-L5601 carrying pJZ065 integrated at the tRNAArg site | 47 |
| DP-L5749 | 10403S carrying pBHE573 | 35 |
| DP-L5750 | 10403S expressing PSA bacteriophage holin/lysin and carrying pBHE573 | 35 |
| DP-L5751 | DP-L5601 carrying pBHE573 | This study |
| DP-L5755 | 10403S carrying pJZ095 integrated at the tRNAArg site | This study |
| EJ-L12 | 10403S ΔinlA | 4 |
| HEL-335 | 10403S plcBΔpro | 46 |
| HEL-981 | NF-L943 Mpl-FlagN-cata | 12 |
| HEL-1216 | DP-L2296 prsA2::Himar1 | This study |
| HEL-1230 | NF-L943 Mpl-FlagN-catprsA2::Himar1 | This study |
| HEL-1232 | NF-L943 Mpl-FlagN-catprsA2::Himar1 carrying pJZ065 integrated at the tRNAArg site | This study |
| HEL-1405 | HEL-335 prsA2::Himar1 | This study |
| NF-L1166 | 10403S with actA-gus-neo-plcB and prfA with the L140F mutation | 30 |
| Plasmids | ||
| pBHE573 | Firefly luciferase with the cytomegalovirus promoter | 35 |
| pJZ065 | prsA2 promoter, without prfA box, and open reading frame cloned into pPL2 | 47 |
| pJZ095 | prsA2 promoter-gus cloned into pPL2 (PPrsA2-gus) | This study |
| pPL2 | Site-specific shuttle integration vector | 22 |
a
Mpl-FlagN-cat is an Mpl construct that contains a Flag tag at the N terminus of its catalytic domain.