Fig. 6.

Q548R IE2 C-F HB5 is not defective in transitioning to late gene expression. HFFs were mock infected or infected with either WT C-F HB5, Q548R IE2 C-F HB5, or Rev-Q548R IE2 C-F HB5 (MOI = 0.5). Cells were harvested at the indicated times. Equivalent amounts of protein were loaded onto an 8% acrylamide gel and then transferred to a nitrocellulose membrane. The membrane was probed with antibodies against UL32 (pp150), UL82 (pp71), UL85 (minor capsid protein), and UL86 (major capsid protein). β-Actin served as a loading control.