Fig. 7.

Q548R IE2 86 interacts with UL84 and is not directly responsible for the decrease in UL84 expression. (A) HFFs were mock infected or infected with either WT C-F HB5 or Q548R IE2 C-F HB5 and were harvested at the indicated times. Cell lysates were then immunoprecipitated with the CH160 antibody. Protein from the pre-IP, IP, and post-IP samples were then examined via Western blotting with the UL84 antibody as well as the CH160 antibody. Pre-IP and post-IP samples represent 10% of the total IP sample. (B) 293FT cells were transfected with equivalent amounts of plasmids expressing the indicated proteins. 86*, Q548R IE2 86; 40*, Q548R IE2 40. Cells were harvested at 48 h p.t. and examined via Western blotting with the monoclonal IE2 antibody and the monoclonal UL84 antibody. β-Actin served as a loading control.