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Comparison of hematoxylin staining, IHC, and ISH of a moderately PCV2-infected spleen section. (a) Hematoxylin-stained section (HE). (b) Anti-PCV2 IHC (F217 monoclonal anti-PCV2 antibody clone [29]). (c) ISH with AB oligonucleotide hybridization against PCV2 DNA. Bars, 20 μm.
