Fig. 9.

PCV2 genotype group colocalization and coreplication visualized in a confocal micrograph. Genotype group superinfection and coreplication were visualized by FISH of a formalin-fixed and paraffin-embedded tonsil tissue section. (a) Red labeling achieved by hybridization of PCV2a dsDNA-specific oligonucleotide Ar. (b) Oligonucleotide Br target hybridization specific for PCV2b dsDNA appears green. (c) Overlay of panels a and b. In all panels, nuclei are counterstained with DAPI and thus appear blue. Bars, 15 μm.