Fig. 1.

In vitro replication and apoptosis induction of ECTV SPI-2 mutants is similar to that of wt virus. (A and B) L929 cells (A) and MEF (B) were infected at an MOI of 3 with ECTV or ECTV SPI-2 mutants. The cell-associated viral load was quantified by plaque assay. The graphs show means ± standard errors of the mean (SEM) of one representative of three independent experiments. (C and D) MEF were infected as for panel B. Infected-cell apoptosis (C) and mitochondrial potential disruption (D) were quantified by phosphatidylserine exposure (annexin⁺ 7AAD⁻) and DiOC₆ staining of MEF, respectively, at the indicated times p.i.