Fig. 1.
Membrane association of the chimeric viroporin 2B/P2 protein. At the top is a schematic representation of the poliovirus 2B/P2 chimeric protein (the fused P2 domain is shown in gray). Amino acid residues are shown (HR1 and HR2 are highlighted in gray boxes). The gel in the middle shows the segregation of [35S]Met/Cys-labeled viroporin 2B/P2 fusion protein into membranous and soluble fractions (untreated) and after alkaline wash (Alk. Ext.; sodium carbonate buffer) or urea treatments. P and S denote pellet and supernatant, respectively. In the graph at the bottom, to calculate the percentages of protein the signals present in each pellet and supernatant pair were summed and set to 100%. Data correspond to averages from at least three independent experiments; error bars show standard deviations.