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. 2011 Nov;85(21):11315–11324. doi: 10.1128/JVI.05421-11

Fig. 2.

Fig. 2.

Triton X-114 partition of viroporin 2B and 2B-derived proteins. (A) Structural organization of the proteins used in the Triton X-114 partition experiments. (B) SDS-PAGE (12% polyacrylamide) analysis after Triton X-114 treatment of 2B/P2 (∼38 kDa) and 2B/50P2 (∼16 kDa) proteins. Integral membrane protein Lep (∼37 kDa; the nonglycosylated form) and peripheral PNRSV movement protein (∼32 kDa) were processed in parallel as control samples. (C) Phase separation of viroporin 2B. As a control for small integral membrane protein, Turnip crinkle virus p9 movement protein (∼9 kDa) was included. The gels used contained 20% polyacrylamide. AP and OP refer to aqueous and organic phases, respectively.