Fig. 5.

Membrane insertion of 60-mer viroporin 2B. (A) Structural organization of the 2B 60-mer truncated construct. (B) In vitro translations were performed in the presence (+) or in the absence of C-terminal glycosylation tag, RMs, and EndoH as indicated. (C) The 2BNt/Ct construct was translated in either the absence (lanes 1 and 3) or the presence (lane 2) of RMs. Lane 2, cotranslationally added microsomes. Lane 3, RMs were added posttranslationally (after 1 h of translation and 10 min of cycloheximide treatment; Post-), and incubation was continued for another 1 h.