Fig. 7.

Insertion and topology of full-length viroporin 2B protein. (A) The structural organization of the 2B-derived constructs is shown at the top. The N-glycosylation site is highlighted by a Y-shaped symbol both when inserted in the protein sequence and when added as a C-terminal reporter tag (rectangles). (B) In vitro translation was performed in the presence (+) and in the absence (−) of RMs and EndoH as indicated. Nonglycosylated and singly glycosylated proteins are indicated by empty and black dots, respectively.