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. 2011 Nov;85(21):10968–10975. doi: 10.1128/JVI.00706-11

Fig. 2.

Fig. 2.

Association of E5 and calpactin I in HPV-16 E6/E7-immortalized HEC lines. (A) Association of ANXA2 with E5. AU1 immunoprecipitates from stable cell lines expressing AU1 epitope-tagged full-length E5 and E5 C-terminal deletion mutants were immunoblotted to detect associated ANXA2 (upper panel; arrow). The immunoblot was relabeled (lower panel) to show levels of E5 proteins in the immunoprecipitates. (B) Association of p11 with ANXA2 by immunoprecipitation and immunoblotting. (C) Association of p11 and E5 by immunoprecipitation and immunoblotting in lysates prepared using modified CHAPS buffer (23). Molecular mass markers (in kilodaltons) are indicated on the left. IP, immunoprecipitation; IB, immunoblotting.