Fig. 2.

Screening of chemotactic factors involved in the migration of hMSCs. The migration of hMSCs to 1% brain extracts of prion-infected mice was assessed after pretreatment of hMSCs with antibodies against receptors for chemokines or growth factors (A) or after pretreatment of brain extracts with antibodies against growth factors or chemokines (B). Migration assays, in which antibody pretreatments of hMSCs and brain extracts were omitted, were assigned as a control (Cont). The graphs show relative numbers of hMSCs that migrated to the brain extracts of prion-infected mice compared to numbers of hMSCs that migrated to brain extracts in control experiments. Means and SDs from three independent assays (each assay was carried out in triplicate) are shown. *, P < 0.05 (Dunnett's post hoc test).