Table 1.

Primers used for BAC recombineering

Knockout primer	Sequence
ToledoΔUL133-138 fw	5′-ATGGGTTGCGACGTGCACGATCCTTCGTGGCAATGCCAATGGGGCGTTCCCGTCGTGGAATGCCTTCGAATTC-3′
ToledoΔUL133-138 rv	5′-GTCAAAGCGACATTATCGCGATCCGCTCCCCTCTTTTTTCTTTTTCTCATACAAGGACGACGACGACAAGTAA-3′
ToledoΔUL139-144 fw	5′-GCTGATTGTTACGACAAACGAGTTGGTATATCCATTATATAGTAACGAACCGTCGTGGAATGCCTTCGAATTC-3′
ToledoΔUL139-144 rv	5′-TTATCTGTGTGACTTCATCGTACCGTGATGTAAAAACAACAACAGGAAGCACAAGGACGACGACGACAAGTAA-3′
ToledoΔUL145-148 fw	5′-TGCAGCTTGGAGTGACTAAAGTGTGTCAGCATAATGAAGTGCAACTGGGCCGTCGTGGAATGCCTTCGAATTC-3′
ToledoΔUL145-148 rv	5′-ATCGCCGCCATCATTGTAATCAGCAATGTGTTGAGGTACTGCACGATGAAACAAGGACGACGACGACAAGTAA-3′
ToledoΔUL148A-D fw	5′-TTGTACCCCCCTTCCCCTCCGTGTTGTAGCCCATCGGCCGCGGCGATCTCCGTCGTGGAATGCCTTCGAATTC-3′
ToledoΔUL148A-D rv	5′-TTTTCCTGTGTTAACACCGGCGTTTTTGGGACGGTCGGTTAACGTGGGTTACAAGGACGACGACGACAAGTAA-3′
ToledoΔUL149-151 fw	5′-CAGTGTTGCTATCGTCATCTTCATCGGAGTCTGTCTGGTGGCCCTGATGTCGTCGTGGAATGCCTTCGAATTC-3′
ToledoΔUL149-151 rv	5′-CATGGTCTTTGTCAGCGGCACGGCGCTGGGGACGGGGTTTCACCGCGCTGACAAGGACGACGACGACAAGTAA-3′