Fig. 2.

Anti-M 5H7-IgA antibodies inhibited MV replication in a dose-dependent manner. Polarized Vero-IgR cells grown in transwells were infected by measles virus at an MOI of 1, and 2 h later, 2.5, 5, 10, 20, or 30 μg of 5H7-IgA or 5H7-IgG MAb was added to the basal chambers. Thirty hours after the MAbs were added, residual antibody was removed by washing the basal surface three times. Fresh medium was added, and 48 h after initial exposure to virus, apical and basal supernatants and cell lysates were collected and virus titers were assessed by plaque assay. The number of PFU per well and the percentage of virus reduction were calculated by comparison with no-antibody control. The percent reduction of virus was calculated over no-antibody control. *, virus titers were significantly lower (P ≤ 0.05) than those from cells exposed to no antibody; ***, P ≤ 0.001. The error bars indicate standard deviations.